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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-117719 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLDIP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.59 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLDIP3 using POLDIP3 Polyclonal Antibody (Product # PA5-117719) at 3.2 µg/mL. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 47, 43 kDa. Observed band size: 43 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of POLDIP3 in HeLa cells using POLDIP3 Polyclonal Antibody (Product # PA5-117719) diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of POLDIP3 in paraffin-embedded human testis tissue using POLDIP3 Polyclonal Antibody (Product # PA5-117719) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating POLDIP3 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: POLDIP3 Polyclonal Antibody (Product # PA5-117719) (8 µg) + Jurkat whole cell lysate (500 µg). Lane 3: Jurkat whole cell lysate (10 µg).
