ALX-804-579-C100

antibody from Enzo Life Sciences

Targeting: SEL1L Hrd3, IBD2, SEL1L1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ALX-804-579-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-579-C100, RRID:AB_2052530

Product name

SEL1L monoclonal antibody (MSel1)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein G affinity purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

MSel1

Vial size

100 μg

Storage

+4°C

Handling

Do not freeze.

References

Submitted references

A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes.

Kaneko M, Yasui S, Niinuma Y, Arai K, Omura T, Okuma Y, Nomura Y
FEBS letters 2007 Nov 27;581(28):5355-60

---

Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells.

Schelhaas M, Malmström J, Pelkmans L, Haugstetter J, Ellgaard L, Grünewald K, Helenius A
Cell 2007 Nov 2;131(3):516-29

---

SEL1L a multifaceted protein playing a role in tumor progression.

Biunno I, Cattaneo M, Orlandi R, Canton C, Biagiotti L, Ferrero S, Barberis M, Pupa SM, Scarpa A, Ménard S
Journal of cellular physiology 2006 Jul;208(1):23-38

---

SEL1L expression in pancreatic adenocarcinoma parallels SMAD4 expression and delays tumor growth in vitro and in vivo.

Cattaneo M, Orlandini S, Beghelli S, Moore PS, Sorio C, Bonora A, Bassi C, Talamini G, Zamboni G, Orlandi R, Ménard S, Bernardi LR, Biunno I, Scarpa A
Oncogene 2003 Sep 25;22(41):6359-68

---

SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro.

Orlandi R, Cattaneo M, Troglio F, Casalini P, Ronchini C, Ménard S, Biunno I
Cancer research 2002 Jan 15;62(2):567-74

---

Production of a monoclonal antibody directed against the recombinant SEL1L protein.

Orlandi R, Cattaneo M, Troglio F, Campiglio M, Biunno I, Ménard S
The International journal of biological markers 2002 Apr-Jun;17(2):104-11

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis using MAb to SEL1L (MSel1) (Prod. No. ALX-804-579).Lane 1: MAb to SEL1L (MSel1)Lane 2: Immunoprecipitation of total lysates from BT-474 cells followed by Western blot analysis using MAb to SEL1L (MSel1) (Prod. No. ALX-804-579) (see method below)Lane 3: No lysateLane 4: MW marker. Method: Cells were lysed using a buffer containing 50mM TRIS-HCl, pH 7.4, 150mM NaCl, 10% glycerol, 0.5% NP40 and protease inhibitors (10µg/ml aproteinin, 1µg/ml leupeptin, 1µg/ml PMSF) for 10 min. at 4¡C. Lysates were then centrifuged at 12'000 rpm for 10 min. at 4¡C to remove insoluble material. Protein concentration was determined by Comassie Plus Protein Assay (Pierce, Rockford, IL). Protein extracts (1.5mg/sample) were immunoprecipitated for 2 hours at 4¡C using 5µg of SEL1L and 20µl of protein-A/G Sepharose beads (Pharmacia-Amersham). Precipitates were then separated on 10% SDS-PAGE, transferred onto nitrocellulose membrane (Amersham) and probed with 10µg/ml MAb to SEL1L (MSel1). Proteins were revealed using ECL-Plus chemiluminescence (Amersham) following the manufacturer's instructions.Note: MAb to SEL1L (MSel1) can recognize isoforms of SEL1L protein with MW lower than 88kDa.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Pancreatic adenocarcinomas: (b) with and (c) without SEL1L expression.