20040-1-AP

antibody from Invitrogen Antibodies

Targeting: MPRIP M-RIP, p116Rip, RHOIP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20040-1-AP

Provider

Invitrogen Antibodies

Product name

MPRIP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody recognizes the C-term of isoform 2 and isoform 3 of MPRIP.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.16 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MPRIP antibody 20040-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MPRIP (IP: 20040-1-AP, 4 µg; Detection: 20040-1-AP 1:500) with SKOV-3 cells lysate 2000 µg.