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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB7585 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7585, RRID:AB_1578122
- Product name
- NOVA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NOVA1.
- Antigen sequence
C-REMPQNVAKTEPVS- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Evolution of Nova-dependent splicing regulation in the brain.
Jelen N, Ule J, Zivin M, Darnell RB
PLoS genetics 2007 Oct;3(10):1838-47
PLoS genetics 2007 Oct;3(10):1838-47
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NOVA1 polyclonal antibody (Cat # PAB7585) (0.01 ug/mL) staining of human breast cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 293 overexpressing NOVA1 and probed with NOVA1 polyclonal antibody (Cat # PAB7585) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NOVA1 polyclonal antibody (Cat # PAB7585) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
