LS-C203255

antibody from LSBio

Targeting: CXCL8 3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C203255

Provider

LSBio

Product name

IL8 / Interleukin 8 Antibody (clone 4H7C7) LS-C203255

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4H7C7

Storage

Store at -20°C. Long term: aliquot and store at -20°C. Reconstituted: store at 2°C to 8°C for up to 3 weeks. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.