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LearnMAB5351-100
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB5351-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-8/CXCL8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha , 12/SDF-1 beta , rhCXCL13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha , rmCXCL13, recombinant rat (rr) CXCL1, rrCXCL3/CINC2 alpha , or rr3/CINC-2 beta is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA43461- Isotype
- IgG
- Antibody clone number
- 105105
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tønnesen E
Intensive care medicine 2007 Jun;33(6):1085-93
Intensive care medicine 2007 Jun;33(6):1085-93
Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Larsson A, Schmitz O, Ørskov H, Tønnesen E
Anesthesiology 2004 Apr;100(4):861-70
Anesthesiology 2004 Apr;100(4):861-70
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL.
