IC208P
antibody from R&D Systems
Targeting: CXCL8
3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC208P - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-8/CXCL8 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL8/IL-8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL-8 and no cross-reactivity with rrCXCL3/CINC-2 alpha .
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P10145
- Isotype
- IgG
- Antibody clone number
- 6217
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references TGFb1 suppresses the activation of distinct dNK subpopulations in preeclampsia.
Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater.
Gene and protein characteristics reflect functional diversity of CD56dim and CD56bright NK cells.
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Zhang J, Dunk CE, Shynlova O, Caniggia I, Lye SJ
EBioMedicine 2019 Jan;39:531-539
EBioMedicine 2019 Jan;39:531-539
Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater.
Baci D, Gallazzi M, Cascini C, Tramacere M, De Stefano D, Bruno A, Noonan DM, Albini A
International journal of molecular sciences 2019 Jan 14;20(2)
International journal of molecular sciences 2019 Jan 14;20(2)
Gene and protein characteristics reflect functional diversity of CD56dim and CD56bright NK cells.
Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R
Journal of leukocyte biology 2006 Dec;80(6):1529-41
Journal of leukocyte biology 2006 Dec;80(6):1529-41
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCL8/IL-8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 PE-conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). Inhibition of IC208P staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.