Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 682902 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#682902, RRID:AB_2566697
- Product name
- Purified anti-Betacellulin
- Antibody type
- Monoclonal
- Antigen
- Purified Human Betacellulin, amino acids (Asp32-Tyr111) (Accession# NM_001729), was expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A15008E
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of recombinant human Betacellulin (lane 1), recombinant mouse Betacellulin (lane 2), A431 (lane 3), MCF-7 (lane 4), and U251 (lane 5). Anti- Betacellulin antibody (clone A15008E) was used, followed by HRP anti-mouse IgG antibody. α-Tubulin antibody (clone AA13) was used as a loading control. Please note this antibody works better under non-reducing conditions for western blotting. Please make sure there is no DTT or beta-Mercaptoethanol in your cell lysate or sample buffer.
- Conjugate
- Unconjugated