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Blocking/NeutralizingAntibody data
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- References [1]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00010535-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010535-M01, RRID:AB_2301181
- Product name
- Mouse Monoclonal RNASEH2A Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNASEH2A - ribonuclease H2, large subunit
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Antiribonuclease H2 antibodies are an immune biomarker for systemic lupus erythematosus.
Nozawa K, Doe K, Uomori K, Sekigawa I, Takasaki Y, Yamaji K, Tamura N
Autoimmunity 2017 Jun;50(4):241-246
Autoimmunity 2017 Jun;50(4):241-246
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Analysis of RNASEH2A expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.
