NB100-86989

antibody from Novus Biologicals

Targeting: WDR20 DMR, FLJ33659, MGC33177

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-86989

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-86989, RRID:AB_2304392

Product name

Rabbit Polyclonal WDR20 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WDR20 Antibody [NB100-86989] - Detection of Human and Mouse WDR20 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-WDR20 antibody NB100-86989 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. WDR20 was also immunoprecipitated by another rabbit anti-WDR20 antibody which recognizes an upstream epitope.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: WDR20 Antibody [NB100-86989] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-WDR20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB