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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF3054 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Versican Isoform V0 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Versican isoform V0 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P13611- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Versican is differentially regulated in the adventitial and medial layers of human vein grafts.
Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting.
Kenagy RD, Kikuchi S, Evanko SP, Ruiter MS, Piola M, Longchamp A, Pesce M, Soncini M, Deglise S, Fiore GB, Haefliger JA, Schmidt TA, Majesky MW, Sobel M, Wight TN
PloS one 2018;13(9):e0204045
PloS one 2018;13(9):e0204045
Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting.
Kischel P, Waltregny D, Dumont B, Turtoi A, Greffe Y, Kirsch S, De Pauw E, Castronovo V
International journal of cancer 2010 Feb 1;126(3):640-50
International journal of cancer 2010 Feb 1;126(3):640-50
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Versican in HeLa Human Cell Line. Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
