OAAB08217

antibody from Aviva Systems Biology

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB08217

Provider

Aviva Systems Biology

Product name

AKT1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

AKT1 Antibody (pT450) (Cat. #OAAB08217) western blot analysis in mouse cerebellum tissue lysates (35µg/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

AKT1 Antibody (C-term T450) (Cat. #OAAB08217)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

AKT1 Antibody (C-term T450) (Cat. #OAAB08217) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.