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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP11976a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP11976a, RRID:AB_10820100
- Product name
- AKT1 Antibody (C-term T450)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- RB29255
- Vial size
- 400 µl
- Storage
- Store at 2 to 8°C.Antibody is stable for 48 months.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AKT1 Antibody (pT450) (Cat. #AP11976a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AKT1 Antibody (pT450) (Cat. #AP11976a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of AKT1 Antibody (C-term T450)(Cat#AP11976a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AKT1 Antibody (C-term T450) (Cat. #AP11976a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AKT1 Antibody (C-term T450) (Cat. #AP11976a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
