GTX110613

antibody from GeneTex

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110613

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110613, RRID:AB_10617128

Product name

AKT1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Copanlisib, a novel phosphoinositide 3-kinase inhibitor, combined with carfilzomib inhibits multiple myeloma cell proliferation.

Okabe S, Tanaka Y, Tauchi T, Ohyashiki K
Annals of hematology 2019 Mar;98(3):723-733

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MicroRNA-296 inhibits colorectal cancer cell growth and enhances apoptosis by targeting ARRB1-mediated AKT activation.

Zhang Z, Zhong X, Xiao Y, Chen C
Oncology reports 2019 Jan;41(1):619-629

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A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells.

Vazquez-Mellado MJ, Aguilar C, Rocha-Zavaleta L
Life sciences 2015 Sep 15;137:142-9

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A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394

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The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.

Chen YJ, Cheng YJ, Hung AC, Wu YC, Hou MF, Tyan YC, Yuan SS
Gynecologic oncology 2013 Dec;131(3):734-43

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE GTX110613 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX110613 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Akt1 antibody detects Akt1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Akt1 protein stained by Akt1 antibody (GTX110613) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments.IP samples: 30 £gg whole cell extract of Akt1-transfected 293T cells.A. 30 £gg whole cell extract of Akt1-protein expressing 293T cellB. Control with 3 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Akt1 protein by 3 £gg Akt1 antibody (GTX110613)10 % SDS-PAGEThe immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1(GTX110613) antibody at 1:250 dilution.