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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [42]
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- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX121937 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121937, RRID:AB_10723121
- Product name
- AKT antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
DSE promotes aggressive glioma cell phenotypes by enhancing HB-EGF/ErbB signaling.
Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.
A high-throughput pipeline for validation of antibodies.
Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.
Activation of fibroblasts by nicotine promotes the epithelial-mesenchymal transition and motility of breast cancer cells.
Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.
Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
AKT and its related molecular feature in aged mice skin.
MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Hydrogen Sulphide Treatment Increases Insulin Sensitivity and Improves Oxidant Metabolism through the CaMKKbeta-AMPK Pathway in PA-Induced IR C2C12 Cells.
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
The effects of artocarpin on wound healing: in vitro and in vivo studies.
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Macrophages induce AKT/β-catenin-dependent Lgr5(+) stem cell activation and hair follicle regeneration through TNF.
N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Targeting insulin-like growth factor-binding protein-3 by microRNA-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer.
The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation.
Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.
Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Epithelial to mesenchymal transition in the liver field: the double face of Everolimus in vitro.
Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor.
FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Heparin co-factor II enhances cell motility and promotes metastasis in non-small cell lung cancer.
p22phox confers resistance to cisplatin, by blocking its entry into the nucleus.
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
GALNT2 suppresses malignant phenotypes through IGF-1 receptor and predicts favorable prognosis in neuroblastoma.
C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization.
Huang C, Huang YL, Wang CC, Pan YL, Lai YH, Huang HC
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Lai YJ, Yu WN, Kuo SC, Ho CT, Hung CM, Way TD, Chen CT
Journal of cellular physiology 2019 Jun;234(6):9118-9129
Journal of cellular physiology 2019 Jun;234(6):9118-9129
Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Li HY, Leu YL, Wu YC, Wang SH
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Ko PH, Shen YC, Murugan K, Huang CW, Sivakumar G, Pal P, Liao CC, Luo KS, Chuang EY, Tsai MH, Lai LC
Scientific reports 2019 Feb 14;9(1):2147
Scientific reports 2019 Feb 14;9(1):2147
DSE promotes aggressive glioma cell phenotypes by enhancing HB-EGF/ErbB signaling.
Liao WC, Liao CK, Tsai YH, Tseng TJ, Chuang LC, Lan CT, Chang HM, Liu CH
PloS one 2018;13(6):e0198364
PloS one 2018;13(6):e0198364
Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.
Chen ST, Kuo TC, Liao YY, Lin MC, Tien YW, Huang MC
Oncogene 2018 Nov;37(46):6041-6053
Oncogene 2018 Nov;37(46):6041-6053
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.
Chang HP, Lu CC, Chiang JH, Tsai FJ, Juan YN, Tsao JW, Chiu HY, Yang JS
International journal of oncology 2018 May;52(5):1504-1514
International journal of oncology 2018 May;52(5):1504-1514
Activation of fibroblasts by nicotine promotes the epithelial-mesenchymal transition and motility of breast cancer cells.
Chen PC, Lee WY, Ling HH, Cheng CH, Chen KC, Lin CW
Journal of cellular physiology 2018 Jun;233(6):4972-4980
Journal of cellular physiology 2018 Jun;233(6):4972-4980
Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.
Viedma-Rodríguez R, Martínez-Hernández MG, Flores-López LA, Baiza-Gutman LA
Molecular and cellular biochemistry 2018 Jan;437(1-2):65-80
Molecular and cellular biochemistry 2018 Jan;437(1-2):65-80
Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
Wu PY, You YJ, Liu YJ, Hou CW, Wu CS, Wen KC, Lin CY, Chiang HM
International journal of molecular sciences 2018 Apr 7;19(4)
International journal of molecular sciences 2018 Apr 7;19(4)
AKT and its related molecular feature in aged mice skin.
Chen H, Wang X, Han J, Fan Z, Sadia S, Zhang R, Guo Y, Jiang Y, Wu Y
PloS one 2017;12(6):e0178969
PloS one 2017;12(6):e0178969
MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Liu J, Cao L, Feng Y, Li Y, Li T
Biotechnology letters 2017 Sep;39(9):1343-1349
Biotechnology letters 2017 Sep;39(9):1343-1349
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Wang JP, Hsieh CH, Liu CY, Lin KH, Wu PT, Chen KM, Fang K
Oncology letters 2017 Sep;14(3):3503-3509
Oncology letters 2017 Sep;14(3):3503-3509
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Ni YL, Hsieh CH, Kim SH, Wang JP, Su CL, Yao CF, Fang K
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Hydrogen Sulphide Treatment Increases Insulin Sensitivity and Improves Oxidant Metabolism through the CaMKKbeta-AMPK Pathway in PA-Induced IR C2C12 Cells.
Chen X, Zhao X, Lan F, Zhou T, Cai H, Sun H, Kong W, Kong W
Scientific reports 2017 Oct 16;7(1):13248
Scientific reports 2017 Oct 16;7(1):13248
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Liu J, Chen W, Zhang H, Liu T, Zhao L
Oncology letters 2017 Nov;14(5):5711-5718
Oncology letters 2017 Nov;14(5):5711-5718
The effects of artocarpin on wound healing: in vitro and in vivo studies.
Yeh CJ, Chen CC, Leu YL, Lin MW, Chiu MM, Wang SH
Scientific reports 2017 Nov 15;7(1):15599
Scientific reports 2017 Nov 15;7(1):15599
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Chao W, Deng JS, Li PY, Liang YC, Huang GJ
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Macrophages induce AKT/β-catenin-dependent Lgr5(+) stem cell activation and hair follicle regeneration through TNF.
Wang X, Chen H, Tian R, Zhang Y, Drutskaya MS, Wang C, Ge J, Fan Z, Kong D, Wang X, Cai T, Zhou Y, Wang J, Wang J, Wang S, Qin Z, Jia H, Wu Y, Liu J, Nedospasov SA, Tredget EE, Lin M, Liu J, Jiang Y, Wu Y
Nature communications 2017 Mar 27;8:14091
Nature communications 2017 Mar 27;8:14091
N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Kuo YH, Chen CC, Wu PY, Wu CS, Sung PJ, Lin CY, Chiang HM
BMC complementary and alternative medicine 2017 Jan 23;17(1):71
BMC complementary and alternative medicine 2017 Jan 23;17(1):71
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414
Oncogene 2017 Aug;36(31):4405-4414
The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways.
Chen X, Zhao X, Cai H, Sun H, Hu Y, Huang X, Kong W, Kong W
Redox biology 2017 Aug;12:987-1003
Redox biology 2017 Aug;12:987-1003
Targeting insulin-like growth factor-binding protein-3 by microRNA-125b promotes tumor invasion and poor outcomes in non-small-cell lung cancer.
Wang HH, Wang YC, Wu DW, Hung CS, Chen CY, Lee H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Apr;39(4):1010428317694316
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Apr;39(4):1010428317694316
The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.
Chen PH, Shen WL, Shih CM, Ho KH, Cheng CH, Lin CW, Lee CC, Liu AJ, Chen KC
Neuropharmacology 2017 Apr;116:300-314
Neuropharmacology 2017 Apr;116:300-314
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Chen CC, Lin MW, Liang CJ, Wang SH
PloS one 2016;11(7):e0158662
PloS one 2016;11(7):e0158662
A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
Hsieh CH, Lee LC, Leong WY, Yang TC, Yao CF, Fang K
Drug design, development and therapy 2016;10:2947-2957
Drug design, development and therapy 2016;10:2947-2957
The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Chen PH, Chang CK, Shih CM, Cheng CH, Lin CW, Lee CC, Liu AJ, Ho KH, Chen KC
Neuropharmacology 2016 Nov;110(Pt A):362-375
Neuropharmacology 2016 Nov;110(Pt A):362-375
Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Lin CS, Lee HT, Lee MH, Pan SC, Ke CY, Chiu AW, Wei YH
International journal of molecular sciences 2016 May 25;17(6)
International journal of molecular sciences 2016 May 25;17(6)
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58
Oncotarget 2016 Feb 2;7(5):6146-58
Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation.
Vallejo-Flores G, Torres J, Sandoval-Montes C, Arévalo-Romero H, Meza I, Camorlinga-Ponce M, Torres-Morales J, Chávez-Rueda AK, Legorreta-Haquet MV, Fuentes-Pananá EM
BioMed research international 2015;2015:761501
BioMed research international 2015;2015:761501
Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.
Huang RF, Wei YJ, Inbaraj BS, Chen BH
International journal of nanomedicine 2015;10:2823-46
International journal of nanomedicine 2015;10:2823-46
Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Chen CY, Lin YS, Chen CL, Chao PZ, Chiou JF, Kuo CC, Lee FP, Lin YF, Sung YH, Lin YT, Li CF, Chen YJ, Chen CH
Oncotarget 2015 Sep 29;6(29):26946-59
Oncotarget 2015 Sep 29;6(29):26946-59
Epithelial to mesenchymal transition in the liver field: the double face of Everolimus in vitro.
Masola V, Carraro A, Zaza G, Bellin G, Montin U, Violi P, Lupo A, Tedeschi U
BMC gastroenterology 2015 Sep 14;15:118
BMC gastroenterology 2015 Sep 14;15:118
Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor.
Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli AM, Gambacurta A
Cell death & disease 2015 Nov 12;6(11):e1974
Cell death & disease 2015 Nov 12;6(11):e1974
FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis.
Huang YL, Chou WC, Hsiung CN, Hu LY, Chu HW, Shen CY
Human molecular genetics 2015 Jun 15;24(12):3506-17
Human molecular genetics 2015 Jun 15;24(12):3506-17
Heparin co-factor II enhances cell motility and promotes metastasis in non-small cell lung cancer.
Liao WY, Ho CC, Hou HH, Hsu TH, Tsai MF, Chen KY, Chen HY, Lee YC, Yu CJ, Lee CH, Yang PC
The Journal of pathology 2015 Jan;235(1):50-64
The Journal of pathology 2015 Jan;235(1):50-64
p22phox confers resistance to cisplatin, by blocking its entry into the nucleus.
Hung CC, Chien CY, Chiang WF, Lin CS, Hour TC, Chen HR, Wang LF, Ko JY, Chang CH, Chen JY
Oncotarget 2015 Feb 28;6(6):4110-25
Oncotarget 2015 Feb 28;6(6):4110-25
HOXA10 is associated with temozolomide resistance through regulation of the homologous recombinant DNA repair pathway in glioblastoma cell lines.
Kim JW, Kim JY, Kim JE, Kim SK, Chung HT, Park CK
Genes & cancer 2014 May;5(5-6):165-174
Genes & cancer 2014 May;5(5-6):165-174
Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
Sun W, Depping R, Jelkmann W
Cell death & disease 2014 Jan 23;5:e1020
Cell death & disease 2014 Jan 23;5:e1020
GALNT2 suppresses malignant phenotypes through IGF-1 receptor and predicts favorable prognosis in neuroblastoma.
Ho WL, Chou CH, Jeng YM, Lu MY, Yang YL, Jou ST, Lin DT, Chang HH, Lin KH, Hsu WM, Huang MC
Oncotarget 2014 Dec 15;5(23):12247-59
Oncotarget 2014 Dec 15;5(23):12247-59
C1GALT1 enhances proliferation of hepatocellular carcinoma cells via modulating MET glycosylation and dimerization.
Wu YM, Liu CH, Huang MJ, Lai HS, Lee PH, Hu RH, Huang MC
Cancer research 2013 Sep 1;73(17):5580-90
Cancer research 2013 Sep 1;73(17):5580-90
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: 293T 7.5% SDS PAGE GTX121937 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of HA-human Akt-transfected 293T cells7.5 % SDS-PAGEAkt1/2/3 antibody (GTX121937) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE GTX121937 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX121937 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Akt1 protein stained by Akt1/2/3 antibody (GTX121937) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 £gg of Akt1/2/3 antibody [N3C2], Internal (GTX121937).Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody (GTX121937).
