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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- TA504234 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504234, RRID:AB_11125764
- Product name
- AKT1 mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)
- Antibody type
- Monoclonal
- Description
- AKT1 mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AKT1
- Isotype
- IgG
- Antibody clone number
- OTI4E10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Insulin-induced endothelial cell cortical actin filament remodeling: a requirement for trans-endothelial insulin transport.
Wang H, Wang AX, Barrett EJ
Molecular endocrinology (Baltimore, Md.) 2012 Aug;26(8):1327-38
Molecular endocrinology (Baltimore, Md.) 2012 Aug;26(8):1327-38
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AKT1 mouse monoclonal antibody (TA504234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunocytochemistry of AKT1 protein level by using anti-AKT1 antibody in bovine aortic endothelial cells (bAEC).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-AKT1 antibody(TA504234),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
