EB07246

antibody from Everest Biotech

Targeting: NONO NMT55, NRB54, P54, P54NRB, PPP1R114

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

EB07246

Provider

Everest Biotech

Proper citation

Everest Biotech Cat#EB07246, RRID:AB_2152157

Product name

Goat anti-NONO / p54NRB Antibody

Antibody type

Polyclonal

Description

Goat anti-NONO / p54NRB Antibody

Reactivity

Human, Mouse

Host

Goat

Antigen sequence

C-NRAAPGAEFAPNK


Epitope

C Terminus

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07246 (0.1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

HEK293 overexpressing Human NONO (RC206688) and probed with EB07246 (mock transfection in first lane), tested by Origene.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07246 (3.8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.