Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- H00006470-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006470-M01, RRID:AB_2188449
- Product name
- Mouse Monoclonal SHMT1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SHMT1 - serine hydroxymethyltransferase 1 (soluble)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, Perco P, PelĂ M, Guerrini R, Amoroso MR, Esposito F, Martello A, Ponterini G, D'Arca D, Costi MP
Journal of proteome research 2014 Nov 7;13(11):5250-61
Journal of proteome research 2014 Nov 7;13(11):5250-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody (M01), clone 4F9.Lane 1: SHMT1 transfected lysate(53.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHMT1 Antibody (4F9) [H00006470-M01] - SHMT1 monoclonal antibody (M01), clone 4F9 Analysis of SHMT1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SHMT1 Antibody (4F9) [H00006470-M01] - Detection limit for recombinant GST tagged SHMT1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHMT1 Antibody (4F9) [H00006470-M01] - Analysis of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.