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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2959 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2959, RRID:AB_1570850
- Product name
- ACAT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ACAT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Androgen-mediated cholesterol metabolism in LNCaP and PC-3 cell lines is regulated through two different isoforms of acyl-coenzyme A:Cholesterol Acyltransferase (ACAT).
Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1.
Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.
Locke JA, Wasan KM, Nelson CC, Guns ES, Leon CG
The Prostate 2008 Jan 1;68(1):20-33
The Prostate 2008 Jan 1;68(1):20-33
Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1.
Guo ZY, Chang CC, Chang TY
Biochemistry 2007 Sep 4;46(35):10063-71
Biochemistry 2007 Sep 4;46(35):10063-71
Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.
Haapalainen AM, Meriläinen G, Pirilä PL, Kondo N, Fukao T, Wierenga RK
Biochemistry 2007 Apr 10;46(14):4305-21
Biochemistry 2007 Apr 10;46(14):4305-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACAT1 polyclonal antibody (Cat # PAB2959) in SK-BR-3 cell line lysates (35 ug/lane).ACAT1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ACAT1 polyclonal antibody (Cat # PAB2959) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
