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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- GTX108471 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108471, RRID:AB_2036696
- Product name
- BCA1 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references ABCG1 is required for pulmonary B-1 B cell and natural antibody homeostasis.
Baldan A, Gonen A, Choung C, Que X, Marquart TJ, Hernandez I, Bjorkhem I, Ford DA, Witztum JL, Tarling EJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 1;193(11):5637-48
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 1;193(11):5637-48
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 15% SDS PAGE GTX108471 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (60 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BCA1 antibody [N3C3] detects secreted BCA1 protein in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
