Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 66113-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AFM Monoclonal Antibody (2G9F6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: SNYDGCCEG DVVQCIRDTS KVMNHICSKQ DSISSKIKEC CEKKIPERGQ CIINSNKDDR PKDLSLREGK FTDSENVCQE RDADPDTFFA KFTFEYSRRH PDLSIPELLR IVQIYKDLLR NCCNTENPPG CYRYAEDKFN ETTEKSLKMV QQECKHFQNL GKDGLKYHYL IRLTKIAPQL STEELVSLGE KMVTAFTTCC TLSEEFACVD NLADLVFGEL CGVNENRTIN PAVDHCCKTN FAFRRPCFES LKADKTYVPP PFSQDLFTFH ADMCQSQNEE LQRKTDRFLV NLVKLKHELT DEELQSLFTN FANVVDKCCK AESPEVCFNE ESPKIGN (264-599 aa encoded by BC109020 )
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G9F6
- Vial size
- 150 µL
- Concentration
- 0.55 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human plasma tissue were subjected to SDS PAGE followed by western blot with 66113-1-IG (AFM Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 66113-1-IG (AFM antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 66113-1-IG ( AFM Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-AFM (IP:66113-1-IG, 5ug; Detection:66113-1-IG 1:500) with human plasma tissue lysate 4000ug.