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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB14791P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-27 R alpha/WSX-1/TCCR PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-27 R alpha/WSX-1/TCCR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human gp130 or recombinant mouse IL-27 Ra is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q6UWB1- Isotype
- IgG
- Antibody clone number
- 191106
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS.
The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies.
IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations.
IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice.
IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-γ release by autologous NK cells.
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
A soluble form of IL-27Rα is a natural IL-27 antagonist.
Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
IL-27-induced gene expression is downregulated in HIV-infected subjects.
Goovaerts O, Massinga-Loembé M, Ondoa P, Ceulemans A, Worodria W, Mayanja-Kizza H, Colebunders R, Kestens L, TB-IRIS Study Group.
PloS one 2019;14(4):e0215991
PloS one 2019;14(4):e0215991
The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies.
Rolvering C, Zimmer AD, Ginolhac A, Margue C, Kirchmeyer M, Servais F, Hermanns HM, Hergovits S, Nazarov PV, Nicot N, Kreis S, Haan S, Behrmann I, Haan C
Journal of leukocyte biology 2018 Nov;104(5):969-985
Journal of leukocyte biology 2018 Nov;104(5):969-985
IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations.
Xu F, Yi J, Wang Z, Hu Y, Han C, Xue Q, Zhang X, Luan X
Immunologic research 2017 Aug;65(4):903-912
Immunologic research 2017 Aug;65(4):903-912
IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice.
Vijayan D, Mohd Redzwan N, Avery DT, Wirasinha RC, Brink R, Walters G, Adelstein S, Kobayashi M, Gray P, Elliott M, Wong M, King C, Vinuesa CG, Ghilardi N, Ma CS, Tangye SG, Batten M
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3008-3017
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3008-3017
IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-γ release by autologous NK cells.
Morandi F, Airoldi I, Pistoia V
Journal of immunology research 2014;2014:938561
Journal of immunology research 2014;2014:938561
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Morandi F, Di Carlo E, Ferrone S, Petretto A, Pistoia V, Airoldi I
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2634-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2634-42
A soluble form of IL-27Rα is a natural IL-27 antagonist.
Dietrich C, Candon S, Ruemmele FM, Devergne O
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5382-9
Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
Chen Q, Swaminathan S, Yang D, Dai L, Sui H, Yang J, Hornung RL, Wang Y, Huang da W, Hu X, Lempicki RA, Imamichi T
PloS one 2013;8(3):e59194
PloS one 2013;8(3):e59194
IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
Kachroo P, Lee MH, Zhang L, Baratelli F, Lee G, Srivastava MK, Wang G, Walser TC, Krysan K, Sharma S, Dubinett SM, Lee JM
Journal of experimental & clinical cancer research : CR 2013 Nov 25;32:97
Journal of experimental & clinical cancer research : CR 2013 Nov 25;32:97
Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
Tzeng TT, Tsay HJ, Chang L, Hsu CL, Lai TH, Huang FL, Shiao YJ
Journal of biomedical science 2013 Dec 6;20:90
Journal of biomedical science 2013 Dec 6;20:90
IL-27-induced gene expression is downregulated in HIV-infected subjects.
Guzzo C, Hopman WM, Che Mat NF, Wobeser W, Gee K
PloS one 2012;7(9):e45706
PloS one 2012;7(9):e45706
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-27 R alpha/WSX-1/TCCR in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IL-27 R alpha/WSX-1/TCCR PE-conjugated Monoclonal Antibody (Catalog # FAB14791P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
