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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Laminin beta-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, NIH-3T3.
- Concentration
- 0.65 mg/mL
Submitted references Cell-Matrix Interactions Contribute to Barrier Function in Human Colon Organoids.
CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.
Varani J, McClintock SD, Aslam MN
Frontiers in medicine 2022;9:838975
Frontiers in medicine 2022;9:838975
CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.
Shaw L, Williams RL, Hamill KJ
BMC research notes 2020 Feb 21;13(1):90
BMC research notes 2020 Feb 21;13(1):90
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin beta 1 using 30 µg of H1299 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Laminin beta 1 polyclonal antibody (Product # PA5-27271) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Laminin beta-1 Polyclonal Antibody (Product # PA5-27271). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 5% SDS PAGE. Laminin beta-1 Polyclonal Antibody (Product # PA5-27271) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Laminin beta-1 Polyclonal Antibody (Product # PA5-27271). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta-1 Polyclonal Antibody (Product # PA5-27271) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Laminin beta 1 in paraformaldehyde-fixed HeLa cells using a Laminin beta 1 polyclonal antibody (Product # PA5-27271) at a 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Laminin beta 1 (Product # PA5-27271) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effects of Aquamin(r) on expression of Laminin beta1: Western blotting. Protein isolated from control and Aquamin(r)-treated (healthy normal subjects) colon organoid-derived monolayer cells was assessed for laminin beta1 expression by Western blotting as described in the Materials and Methods section. 10 mug of protein from each condition was used. beta-actin was assessed in parallel (as a loading control). Band quantitation was done using ImageJ software. Relative band density is presented for laminin beta1 and beta-actin.
