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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00007727-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007727-M01, RRID:AB_2257493
- Product name
- Mouse Monoclonal ZNF174 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ZNF174 - zinc finger protein 174
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ZNF174 Antibody (3E4) [H00007727-M01] - Detection limit for recombinant GST tagged ZNF174 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.
