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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-813B-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-813B-C100, RRID:AB_2052112
- Product name
- Osteoprotegerin (human) monoclonal antibody (Bony-1) (biotin conjugate)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- Bony-1
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Do not freeze. Protect from light.
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- Enzo Life Sciences (provider)
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- Experimental details
- FACS analysis of cells with MAb to OPG (human) (Bony-1) (Prod. No. ALX-804-813). Method: HEK 293T Cells (5 x 10E5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to OPG (human) (Bony-1) (Prod. No. ALX-804-813). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
