- MA5-26999 - Invitrogen Antibodies MST1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of MST1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MST1 (Product # MA5-26999) monoclonal antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-MST1 Monoclonal Antibody (OTI1A10) (Product # MA5-26999) and a 57kDa band corresponding to MST1 was observed across the cell lines and tissues tested except U-87 MG, THP-1 and Mouse Testis. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Caco-2 (Lane 2), U-87 MG (Lane 3), THP-1 (Lane 4), Mouse Liver (Lane 5), Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of MST1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with MST1 Monoclonal Antibody (OTI1A10) (Product # MA5-26999) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane and cytoplasm localization. Panel e represents U-87 MG cells showing no expression of MST1. Panel f represents control Hep G2 cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on paraffin-embedded human tonsil tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a MST1 monoclonal antibody (Product # MA5-26999) at a dilution of 1:150.

MA5-26999