Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF352 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MSP/MST1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human HGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA59872- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fibulin-6 regulates pro-fibrotic TGF-β responses in neonatal mouse ventricular cardiac fibroblasts.
Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
Scientific reports 2017 Feb 17;7:42725
Scientific reports 2017 Feb 17;7:42725
Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
Rampino T, Collesi C, Gregorini M, Maggio M, Soccio G, Guallini P, Dal Canton A
Journal of the American Society of Nephrology : JASN 2002 Mar;13(3):649-57
Journal of the American Society of Nephrology : JASN 2002 Mar;13(3):649-57
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody. Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF352). The ND50 is typically 0.05-0.2 µg/mL.
