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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX105824 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105824, RRID:AB_1950398
- Product name
- GIT1 antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Rho GTPase-independent regulation of mitotic progression by the RhoGEF Net1.
Menon S, Oh W, Carr HS, Frost JA
Molecular biology of the cell 2013 Sep;24(17):2655-67
Molecular biology of the cell 2013 Sep;24(17):2655-67
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis.A. 30 ?g Rat2 whole cell lysate/extract7.5% SDS-PAGEGIT1 antibody [N3C2], Internal (GTX105824) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:Hep G2(GTX27900)7.5% SDS PAGEGTX105824 diluted at 1:2000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (GTX105824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX105824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using GIT1(GTX105824) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1(GTX105824) antibody at 1:100 dilution.
