Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF8508 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GIT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human GIT1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human GIT1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.025 µg/mL of Rabbit Anti-Human GIT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8508) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for GIT1 at approximately 95 & 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GIT1 in Human Brain. GIT1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Rabbit Anti-Human GIT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8508) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
