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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-12982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GIT1 (Tyr554) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology. PA5-12982 was used in western blot to show induction of phophorylation of GIT1 on Y554 in lysates of human umbilical vein endothelial cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of phosphorylated GIT1 was performed by loading human umbilical vein endothelial cell lysates with and without shear stress (at 10 dyn/cm^2) per well onto an SDS PAGE gel. Proteins were transferred to a membrane and blocked with SuperBlock T20 (TBS) Blocking Buffer. The membrane was probed with a Phospho-GIT1 pTyr554 Antibody (Product # PA5-12982) at a dilution of 1:200, washed, and probed with a HRP-conjugated secondary antibody for chemiluminescent detection. β-actin detection (lower panel) was used as loading control. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-GIT1 pTyr554 polyclonal antibody (Product # PA5-12982) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-GIT1 pTyr554 polyclonal antibody (Product # PA5-12982) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.
