GTX106209

antibody from GeneTex

Targeting: ENPP2 ATX, PD-IALPHA, PDNP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX106209

Provider

GeneTex

Proper citation

GeneTex Cat#GTX106209, RRID:AB_1950203

Product name

Autotaxin antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

High Glucose Induces VEGF-C Expression via the LPA1/3-Akt-ROS-LEDGF Signaling Axis in Human Prostate Cancer PC-3 Cells.

Huang YL, Lin YC, Lin CC, Chen WM, Chen BPC, Lee H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(2):597-611

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Activation of Adenylyl Cyclase Causes Stimulation of Adenosine Receptors.

Pleli T, Mondorf A, Ferreiros N, Thomas D, Dvorak K, Biondi RM, Heringdorf DMZ, Zeuzem S, Geisslinger G, Zimmermann H, Waidmann O, Piiper A
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2516-2528

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Association of autotaxin and lysophosphatidic acid receptor 3 with aggressiveness of human breast carcinoma.

Popnikolov NK, Dalwadi BH, Thomas JD, Johannes GJ, Imagawa WT
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2012 Dec;33(6):2237-43

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Autotaxin antibody detects ENPP2 protein by western blot analysis.A. 30 ?g U87-MG whole cell lysate/extract5% SDS-PAGEAutotaxin antibody (GTX106209) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Autotaxin antibody detects ENPP2 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract 7.5% SDS-PAGEAutotaxin antibody (GTX106209) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Autotaxin antibody detects ENPP2 protein by western blot analysis.A. 30 ?g Rat-2 whole cell lysate/extract5% SDS-PAGEAutotaxin antibody (GTX106209) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Human cerebrospinal fluid (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Human cerebrospinal fluid (15 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Autotaxin protein stained by Autotaxin antibody (GTX106209) diluted at 1:500.Blue: Hoechst 33342 staining.