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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX103015 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103015, RRID:AB_1241113
- Product name
- NNT antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- NNT antibody detects NNT protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract | D. 30 ?g A375 whole cell lysate/extract7.5% SDS-PAGENNT antibody (GTX103015) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody (GTX103015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NNT protein stained by NNT antibody (GTX103015) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NNT(GTX103015) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NNT antibody detects NNT protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NNT antibody (GTX103015) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NNT antibody detects NNT protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NNT antibody (GTX103015) dilution: 1:500.
