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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00023530-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00023530-M01, RRID:AB_425908
- Product name
- NNT monoclonal antibody (M01), clone 1D6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NNT.
- Antigen sequence
MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINL
DNAIDMIREANSIIITPGYGLCAAKAQYPIADLVK
MLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPY
DIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQED
PNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDN
PIFYKPNTAMLLGDAKKTCDALQAKVRESYQK- Isotype
- IgG
- Antibody clone number
- 1D6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Ethanol intoxication increases hepatic N-lysyl protein acetylation.
Picklo MJ Sr
Biochemical and biophysical research communications 2008 Nov 21;376(3):615-9
Biochemical and biophysical research communications 2008 Nov 21;376(3):615-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NNT monoclonal antibody (M01), clone 1D6 Western Blot analysis of NNT expression in HepG2 ( Cat # L019V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NNT is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NNT on HepG2 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
