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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 459170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NNT Monoclonal Antibody (8B4BB10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive control: Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8B4BB10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The species specificity was tested with western blot using 5mg/lane BHM, 10mg/lane HHM, HLM, RLM, MLM, and 20mg/lane HepG2 cells as materials. CII-Ip 30kDa subunit mAb was used as internal standard since it works with all the tested species. The high background of rat/mouse sample is due to minimum amount of immunoglobulin in the samples which reacts with 2nd antibody (goat-anti-mouse) directly.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NNT in HepG2 whole cell lysate using an NNT Monoclonal antibody (Product # 459170) at a concentration of 0.5 µg/mL. Predicted band size: 114 kDa. Anti-Complex II 30 kDa subunit has also been labeled as a control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NNT in isolated mitochondria using an NNT Monoclonal antibody (Product # 459170). Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The species specificity was tested with western blot using 5mg/lane BHM, 10mg/lane HHM, HLM, RLM, MLM, and 20mg/lane HepG2 cells as materials. CII-Ip 30kDa subunit mAb was used as internal standard since it works with all the tested species. The high background of rat/mouse sample is due to minimum amount of immunoglobulin in the samples which reacts with 2nd antibody (goat-anti-mouse) directly.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NNT in Human skin fibroblasts using an NNT Monoclonal antibody (Product # 459170). Cultured, normal Human skin fibroblasts (strain HDFn) were labeled with an NNT Monoclonal antibody (Product # 459170) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNT in Human cerebellum using an NNT Monoclonal antibody (Product # 459170) at a dilution of 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NNT in HL-60 cells using an NNT monoclonal antibody (Product # 459170) at 1µg/mL is depicted by the blue line. The red line indicates an isotype control antibody.
