600-401-691

antibody from Rockland Immunochemicals, Inc.

Targeting: PARK7 DJ-1, DJ1, GATD2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-691

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-691, RRID:AB_2299280

Product name

Anti-Human PARK-7 (RABBIT) Antibody - 600-401-691

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis is shown using Rockland's Affinity Purified anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 ?m nitrocellu-lose. The blot was incubated with a 1:1,300 dilution of the antibody overnight at 4° C followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:5,000 for 45 min at RT. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot