AP09257PU-N

antibody from Acris Antibodies GmbH

Targeting: PARK7 DJ-1, DJ1, GATD2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09257PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09257PU-N, RRID:AB_2035416

Product name

anti DJ-1 / PARK7 (177-189)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding aa 177-189 of Human PARK7 protein

Reactivity

Human, Simian, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis is shown using anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 μm nitrocellulose. The blot was incubated with a 1:1,300 dilution of the antibody overnight at 4° C followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-PARK7 antibody was used at a 5 μg/ml to detect PARK7 in a variety of tissues. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows PARK7 staining of human pancreas. Tissue was formalinfixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.