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Western blot
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- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
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- Product number
- NB100-1194 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1194, RRID:AB_2160282
- Product name
- Goat Polyclonal Park7/DJ-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Variants (NP_001116849.1
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Decline in DJ-1 and decreased nuclear translocation of Nrf2 in Fuchs endothelial corneal dystrophy.
BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity.
Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains.
Turning point in apoptosis/necrosis induced by hydrogen peroxide.
Induction of reactive oxygen species by bisphenol A and abrogation of bisphenol A-induced cell injury by DJ-1.
Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons.
DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras.
Bitar MS, Liu C, Ziaei A, Chen Y, Schmedt T, Jurkunas UV
Investigative ophthalmology & visual science 2012 Aug 24;53(9):5806-13
Investigative ophthalmology & visual science 2012 Aug 24;53(9):5806-13
BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity.
Deeg S, Gralle M, Sroka K, Bähr M, Wouters FS, Kermer P
The Journal of cell biology 2010 Feb 22;188(4):505-13
The Journal of cell biology 2010 Feb 22;188(4):505-13
Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains.
Baulac S, Lu H, Strahle J, Yang T, Goldberg MS, Shen J, Schlossmacher MG, Lemere CA, Lu Q, Xia W
Molecular neurodegeneration 2009 Feb 25;4:12
Molecular neurodegeneration 2009 Feb 25;4:12
Turning point in apoptosis/necrosis induced by hydrogen peroxide.
Saito Y, Nishio K, Ogawa Y, Kimata J, Kinumi T, Yoshida Y, Noguchi N, Niki E
Free radical research 2006 Jun;40(6):619-30
Free radical research 2006 Jun;40(6):619-30
Induction of reactive oxygen species by bisphenol A and abrogation of bisphenol A-induced cell injury by DJ-1.
Ooe H, Taira T, Iguchi-Ariga SM, Ariga H
Toxicological sciences : an official journal of the Society of Toxicology 2005 Nov;88(1):114-26
Toxicological sciences : an official journal of the Society of Toxicology 2005 Nov;88(1):114-26
Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons.
Willis D, Li KW, Zheng JQ, Chang JH, Smit AB, Kelly T, Merianda TT, Sylvester J, van Minnen J, Twiss JL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Jan 26;25(4):778-91
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Jan 26;25(4):778-91
DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras.
Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM, Ariga H
Biochemical and biophysical research communications 1997 Feb 13;231(2):509-13
Biochemical and biophysical research communications 1997 Feb 13;231(2):509-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Park7/DJ-1 Antibody [NB100-1194] - Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
