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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB3995 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Park7/DJ-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human Park7 in Western blots.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q99497- Isotype
- IgG
- Antibody clone number
- 421015
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Park7/DJ-1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, LNCaP human prostate cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Park7/DJ-1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
