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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
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- Product number
- PA5-53941 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COBL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AVVRVSPEVP LQNILPVICA KCEVSPEHVV LLRDNIAGEE LELSKSLNEL GIKELYAWDN RRETFRKSSL GNDETDKEKK KFLGFFKVNK RSNSKGCLT
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COBL in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a COBL Polyclonal Antibody (Product # PA5-53941). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of COBL in human cell line CACO-2 shows positivity in cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a COBL Polyclonal Antibody (Product # PA5-53941).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of COBL in human cell line CACO-2 using a COBL Polyclonal Antibody (Product # PA5-53941) shows localization to nucleoplasm and cell junctions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human cerebral cortex using COBL Polyclonal Antibody (Product # PA5-53941).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human colon using COBL Polyclonal Antibody (Product # PA5-53941).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human heart muscle using COBL Polyclonal Antibody (Product # PA5-53941).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human urinary bladder using a COBL Polyclonal Antibody (Product # PA5-53941) shows strong cytoplasmic and membranous positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human cerebral cortex, colon, heart muscle and smooth muscle using COBL Polyclonal Antibody (Product # PA5-53941) (A) shows similar protein distribution across tissues to an independent COBL Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human smooth muscle using COBL Polyclonal Antibody (Product # PA5-53941) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COBL in human parathyroid gland using COBL Polyclonal Antibody (Product # PA5-53941) shows high expression.
