AMAb91514

antibody from Atlas Antibodies

Targeting: PIK3CA PI3K

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91514

Provider

Atlas Antibodies

Product name

Anti-PIK3CA

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

SYSHAGLSNRLARDNELRENDKEQ


Epitope

Binds to an epitope located within the peptide sequence - as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL8347

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb91514 antibody. Corresponding PIK3CA RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows very weak positivity in myocytes as expected.