NBP1-90114

antibody from Novus Biologicals

Targeting: ARPC1B ARC41, p40-ARC, p41-ARC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-90114

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-90114, RRID:AB_11028537

Product name

Rabbit Polyclonal actin-related protein 2/3 complex subunit 1B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human actin-related protein 2/3 complex subunit 1B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Lanthanum Chloride Impairs Learning and Memory and Induces Dendritic Spine Abnormality by Down-Regulating Rac1/PAK Signaling Pathway in Hippocampus of Offspring Rats.

Sun W, Yang J, Hong Y, Yuan H, Wang J, Zhang Y, Lu X, Jin C, Wu S, Cai Y
Cellular and molecular neurobiology 2020 Apr;40(3):459-475

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Disruption of Thrombocyte and T Lymphocyte Development by a Mutation in ARPC1B.

Somech R, Lev A, Lee YN, Simon AJ, Barel O, Schiby G, Avivi C, Barshack I, Rhodes M, Yin J, Wang M, Yang Y, Rhodes J, Marcus N, Garty BZ, Stein J, Amariglio N, Rechavi G, Wiest DL, Zhang Y
Journal of immunology (Baltimore, Md. : 1950) 2017 Dec 15;199(12):4036-4045

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Isoform diversity in the Arp2/3 complex determines actin filament dynamics.

Abella JV, Galloni C, Pernier J, Barry DJ, Kjær S, Carlier MF, Way M
Nature cell biology 2016 Jan;18(1):76-86

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human cell lines SK-MEL-30 and HEK293 using anti-ARPC1B antibody. Corresponding ARPC1B RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human lymph node and skeletal muscle tissues. Corresponding ARPC1B RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human lung shows weak cytoplasmic positivity in macrophages.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human small intestine shows moderate cytoplasmic positivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.