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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- F49135 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SMAD6 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 358-386 from the human protein was used as the immunogen for this SMAD6 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Store at 4°C for up to one month. For long term, aliquot the SMAD6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SMAD6 antibody western blot analysis in (1) HepG2 and (2) HT29 lysate. Observed molecular weight: 50~60 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human lung lysate with SMAD6 antibody. Observed molecular weight: 50~60 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SMAD6 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
