A304-432A

antibody from Invitrogen Antibodies

Targeting: CELSR1 ADGRC1, CDHF9, FMI2, HFMI2, ME2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A304-432A

Provider

Invitrogen Antibodies

Product name

CELSR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CELSR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CELSR1 antibody A304-432A (lot A304-432A-1) used for IP at 6 µg per reaction. CELSR1 was also immunoprecipitated by rabbit anti-CELSR1 antibody A304-433A. For blotting immunoprecipitated CELSR1, A304-432A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CELSR1 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CELSR1 antibody A304-432A (lot A304-432A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.