11210-1-AP

antibody from Invitrogen Antibodies

Targeting: SUMF2 DKFZp566I1024

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11210-1-AP

Provider

Invitrogen Antibodies

Product name

SUMF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LPLLPLLSL LVGAWLKLGN GQATSMVQLQ GGRFLMGTNS PDSRDGEGPV REATVKPFAI DIFPVTNKDF RDFVREKKYR TEAEMFGWSF VFEDFVSDEL RNKATQPMKS VLWWLPVEKA FWRQPAGPGS GIRERLEHPV LHVSWNDARA YCAWRGKRLP TEEEWEFAAR GGLKGQVYPW GNWFQPNRTN LWQGKFPKGD KAEDGFHGVS PVNAFPAQNN YG (1-221 aa encoded by BC015600)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human spleen tissue were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human lung tissue were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human skin tissue were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed A431 cells using 11210-1-AP (SUMF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11210-1-AP ( SUMF2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11210-1-AP ( SUMF2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:500) with A431 cells lysate 1520ug.