PA5-19955

antibody from Invitrogen Antibodies

Targeting: FEM1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19955

Provider

Invitrogen Antibodies

Product name

F1A alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~70kDa. A suggested positive control is mouse liver tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (A) and rat (B) liver tissue lysates using a F1A alpha polyclonal antibody (Product # PA5-19955) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-F1A alpha Polyclonal Antibody (Product # PA5-19955) and a 70 kDa band corresponding to Protein fem-1 homolog B was observed across Mouse Testis, Mouse Heart and Rat Testis, but not in Mouse Ovary and Rat Ovary. Tissue extracts (30 µg lysate) of Mouse Testis (Lane 1), Mouse Heart (Lane 2), Mouse Brain (Lane 3), Mouse Ovary (Lane 4), Rat Testis (Lane 5) and Rat Ovary (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse liver cells using a F1A alpha polyclonal antibody (Product # PA5-19955) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 20 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 20 µg/mL. Green: F1A alpha Blue: DAPI staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of F1Aα in mouse liver tissue with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 5 µg/mL.