LS-C140560

antibody from LSBio

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C140560

Provider

LSBio

Product name

Anti-c-Kit / CD117 Antibody (phospho-Tyr721) LS-C140560

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Peptide Competition and Stimulation Extracts prepared from Mo7e cells left untreated (1) or treated with 100 ng/mL human SCF for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with the c-Kit [pY721] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to c-Kit [pY721] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of c-Kit [pY721] phosphorylation by the addition of SCF to this cell system.