Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C140560 - Provider product page
- Provider
- LSBio
- Product name
- Anti-c-Kit / CD117 Antibody (phospho-Tyr721) LS-C140560
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Peptide Competition and Stimulation Extracts prepared from Mo7e cells left untreated (1) or treated with 100 ng/mL human SCF for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with the c-Kit [pY721] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to c-Kit [pY721] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of c-Kit [pY721] phosphorylation by the addition of SCF to this cell system.