Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C140561 - Provider product page
- Provider
- LSBio
- Product name
- Anti-c-Kit / CD117 Antibody (phospho-Tyr936) LS-C140561
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Peptide Competition Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE l and transferred to PVDF. Membranes were blocked with a BSA-TBST buffer overnight incubated with c-Kit [pY936] antibody for two hours at RTr, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and detected using the Tropix WesternStar method.