LS-C140561

antibody from LSBio

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C140561

Provider

LSBio

Product name

Anti-c-Kit / CD117 Antibody (phospho-Tyr936) LS-C140561

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Peptide Competition Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE l and transferred to PVDF. Membranes were blocked with a BSA-TBST buffer overnight incubated with c-Kit [pY936] antibody for two hours at RTr, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and detected using the Tropix WesternStar method.