MAB332-100

antibody from Novus Biologicals

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB332-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD117/c-kit Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CD117/c-kit in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross-reactivity with recombinant mouse CD117 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 1.0 µg/mL of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD117/c-kit at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD117/c-kit by Simple Western^TM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Human CD117/c-kit Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD117/c-kit Monoclonal Antibody (Catalog # MAB332). The ND₅₀ is typically 0.5-2.5 µg/mL.