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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM20040AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20040AF-N, RRID:AB_10655050
- Product name
- anti CD117 / c-kit
- Antibody type
- Monoclonal
- Antigen
- MOLM-1 cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A3C6E2
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Immunoprecipitation of c-Kit from HEL cells extract with CD117 antibody AM20040AF-N (Lane 1) or normal Mouse IgG (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with anti-c-Kit polyclonal antibody. HEL crude lysate was resolved in Lane 3.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Flow cytometric analysis of CD117 expression on HEL cells (Left) and Jurkat cells (Right). Open Histogram indicates the reaction of Isotypic control to the cells. Shaded Histogram indicates the reaction of CD117 antibody AM20040AF-N to the cells.
