AF1356

antibody from R&D Systems

Targeting: KIT C-Kit, CD117, PBT, SCFR

Provider product page for AF1356

Western blot

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [30]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [1]

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Product number: AF1356 - Provider product page
Provider: R&D Systems
Product name: Human/Mouse CD117/c-kit Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.
Reactivity: Human, Mouse
Host: Goat
Conjugate: Unconjugated
Antigen sequence: P05532
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

KIT protein structure - AF1356 shown in red.

Submitted references Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Agarwal P, Isringhausen S, Li H, Paterson AJ, He J, Gomariz Á, Nagasawa T, Nombela-Arrieta C, Bhatia R
Cell stem cell 2019 May 2;24(5):769-784.e6

Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis.

Kidoya H, Muramatsu F, Shimamura T, Jia W, Satoh T, Hayashi Y, Naito H, Kunisaki Y, Arai F, Seki M, Suzuki Y, Osawa T, Akira S, Takakura N
Nature communications 2019 Mar 6;10(1):1072

Cardiac Nestin⁺ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells.

Xie D, Liao Y, Wu B, Chen Y, Lin W, Lu D, Gao S, Zhu S, Peng C, Jiang MH
International heart journal 2018;59(1):180-189

Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis.

Saeidi S, Shapouri F, de Iongh RU, Casagranda F, Sutherland JM, Western PS, McLaughlin EA, Familari M, Hime GR
PloS one 2018;13(1):e0190925

Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.

Kawai Y, Oda A, Kanai Y, Goitsuka R
PloS one 2018;13(1):e0190702

Multicolor quantitative confocal imaging cytometry.

Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46

Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures.

Lee MY, Ha SE, Park C, Park PJ, Fuchs R, Wei L, Jorgensen BG, Redelman D, Ward SM, Sanders KM, Ro S
PloS one 2017;12(4):e0176031

Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer.

Harrell JC, Shroka TM, Jacobsen BM
Oncogenesis 2017 Nov 27;6(11):396

Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice.

Luo L, Tang J, Nishi K, Yan C, Dinh PU, Cores J, Kudo T, Zhang J, Li TS, Cheng K
Circulation research 2017 May 26;120(11):1768-1775

Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes.

Feng AL, Xiang YY, Gui L, Kaltsidis G, Feng Q, Lu WY
Diabetologia 2017 Jun;60(6):1033-1042

A transit-amplifying population underpins the efficient regenerative capacity of the testis.

Carrieri C, Comazzetto S, Grover A, Morgan M, Buness A, Nerlov C, O'Carroll D
The Journal of experimental medicine 2017 Jun 5;214(6):1631-1641

Concurrent Isolation of 3 Distinct Cardiac Stem Cell Populations From a Single Human Heart Biopsy.

Monsanto MM, White KS, Kim T, Wang BJ, Fisher K, Ilves K, Khalafalla FG, Casillas A, Broughton K, Mohsin S, Dembitsky WP, Sussman MA
Circulation research 2017 Jul 7;121(2):113-124

Live cell screening platform identifies PPARδ as a regulator of cardiomyocyte proliferation and cardiac repair.

Magadum A, Ding Y, He L, Kim T, Vasudevarao MD, Long Q, Yang K, Wickramasinghe N, Renikunta HV, Dubois N, Weidinger G, Yang Q, Engel FB
Cell research 2017 Aug;27(8):1002-1019

Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice.

Luo L, Urata Y, Yan C, Hasan AS, Goto S, Guo CY, Tou FF, Xie Y, Li TS
PloS one 2016;11(5):e0152179

Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.

Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang QD, Lui KO, Zhou B
Cell research 2016 Jan;26(1):119-30

Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis.

Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D
PloS one 2015;10(9):e0138621

Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium.

Iglesias JM, Cairney CJ, Ferrier RK, McDonald L, Soady K, Kendrick H, Pringle MA, Morgan RO, Martin F, Smalley MJ, Blyth K, Stein T
PloS one 2015;10(3):e0119718

Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.

Shiu J, Piazuelo MB, Ding H, Czinn SJ, Drakes ML, Banerjee A, Basappa N, Kobayashi KS, Fricke WF, Blanchard TG
Mucosal immunology 2015 Sep;8(5):1047-59

Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.

Quijada P, Hariharan N, Cubillo JD, Bala KM, Emathinger JM, Wang BJ, Ormachea L, Bers DM, Sussman MA, Poizat C
The Journal of biological chemistry 2015 Oct 16;290(42):25411-26

c-kit+ cells minimally contribute cardiomyocytes to the heart.

van Berlo JH, Kanisicak O, Maillet M, Vagnozzi RJ, Karch J, Lin SC, Middleton RC, Marbán E, Molkentin JD
Nature 2014 May 15;509(7500):337-41

Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.

Smith AJ, Lewis FC, Aquila I, Waring CD, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison GM
Nature protocols 2014 Jul;9(7):1662-81

Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.

Toko H, Hariharan N, Konstandin MH, Ormachea L, McGregor M, Gude NA, Sundararaman B, Joyo E, Joyo AY, Collins B, Din S, Mohsin S, Uchida T, Sussman MA
The Journal of biological chemistry 2014 Feb 28;289(9):5348-56

Thymosin β4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.

Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
Circulation 2013 Sep 10;128(11 Suppl 1):S32-41

Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.

Fang S, Wei J, Pentinmikko N, Leinonen H, Salven P
PLoS biology 2012;10(10):e1001407

Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.

Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
Blood 2012 Jun 7;119(23):5429-37

Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.

Moon TC, Yoshimura T, Parsons T, Befus AD
Journal of leukocyte biology 2012 Apr;91(4):581-90

Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.

Miyamoto K, Yoshida S, Kawasumi M, Hashimoto K, Kimura T, Sato Y, Kobayashi T, Miyauchi Y, Hoshi H, Iwasaki R, Miyamoto H, Hao W, Morioka H, Chiba K, Kobayashi T, Yasuda H, Penninger JM, Toyama Y, Suda T, Miyamoto T
The Journal of experimental medicine 2011 Oct 24;208(11):2175-81

CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.

Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
International immunology 2009 Aug;21(8):991-1001

Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.

Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 1;177(9):5791-800

Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.

Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 1;177(9):5791-800

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Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human and Mouse CD117/c-kit by Simple Western^TM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.